Supplementary Figure 5: Behavior analysis for miR-137_OE and ΔmiR-137_OE.

a–c. Equivalent levels of locomotion and a trend for anxiety in miR-137_OE as measured by a. Open-field arena paradigm: margin distance: t(12.94) = –0.61, ^nsp = 0.5547, r = 0.17; margin time: t(12.20) = –1.89, ^nsp = 0.083, r = 0.48; center distance: t(13.83) = 2.55,*p = 0.023, r = 0.57; center time: t(12.20) = 1.89, ^nsp = 0.083, r = 0.48; total distance: t(12.89) = 1.31, ^nsp = 0.2117, r = 0.34; horizontal activity: t(13.52) = 1.98, ^nsp = 0.0685, r = 0.47; vertical activity: t(13.15) = 1.36, ^nsp = 0.1952, r = 0.35; stereotype: t(13.87) = 2.13, ^nsp = 0.05167, r = 0.50. b. Light-dark box paradigm: Frequency of exploration: t(9.90) = 0.94, ^nsp = 0.3709, r = 0.29, time spend in light: t(11.88) = 0.60, ^nsp = 0.5585, r = 0.17. c. Elevated-plus maze paradigm. Panel from left to right: time spent in closed or open arm, no significant main effect, F(1, 26) = 2.09, ^nsp = 0.161, t_{Time ΔmiR-137} (13.66) = 42.0, ***p < 0.001, r = 1, t_{Time miR-137} (9.86) = 39.68, ***p < 0.001, r = 1; Frequency of entering did not differ between open-closed arm and the condition, F(1, 26) = 0.40, ^nsp = 0.535, t_{Frequency ΔmiR-137} (11.49) = 2.49, *p < 0.05, r = 0.59, t_{Frequency miR-137} (11.49) = 2.49, *p < 0.05, r = 0.59). Distance and velocity, t_distance(12.98) = 0.36, ^nsp = 0.7259, r = 0.10, t_velocity(12.97) = 0.35, ^nsp = 0.7298, r = 0.10. d. Nociception response, W = 20.5, ^nsp = 0.7471, r = –0.09. e. Freezing response after context and before cue in %, t(41.97) = –1.20, ^nsp = 0.2381, r = 0.18. f. Thigmotaxis, percentage of the trail time and path that were spent in the defined thigmotaxis band (0.8) relative to the diameter of the pool, t_Time(24.07) = –0.83, ^nsp = 0.4165, r = 0.17; t_Path(25.57) = –0.15, ^nsp = 0.8838, r = 0.03. g. Three-chamber social interaction paradigm. Amount of time that the mouse spends to explore, left panel: new versus familiar mouse, F(2, 24) = 22.41, ***p < 0.001, right panel: mouse versus object, F(2, 24) = 45.5, ***p < 0.001. No significant difference for ΔmiR-137_OE and miR-137_OE in both experimental set-ups, F_{familiar mouse} (2, 48) = 2.41, ^nsp = 0.10 and F_{empty cage} (2, 48) = 2.86, ^nsp = 0.0673. Social paradigm for the familiar paradigm: for ΔmiR-137_OE: t_{familiar-middle} (–3.81), **p = 0.0023, t_familiar-new (2.86), *p = 0.0225, t_middle-new (6.67), ***p < 0.001; for miR137_OE: t_{familiar-middle} (–5.89), ***p < 0.001, t_familiar-new (3.44), **p = 0.00574, t_middle-new (9.33), ***p < 0.001; for the empty paradigm for ΔmiR-137_OE: t_empty-middle (–6.06), ***p < 0.001, t_empty-mouse (3.35), **p = 0.0718, t_middle-mouse (9.41), ***p < 0.001; for miR-137_OE: t_empty-middle (–4.03), **p = 0.0013, t_empty-mouse (3.19), *p = 0.0106, t_middle-mouse (7.22), ***p < 0.001, n: animals used for each experiment. ns: not significant, s.e.m.