Supplementary Figure 1: Human induced neurons.
From: The schizophrenia risk gene product miR-137 alters presynaptic plasticity
a. Transcriptional luciferase assay for the four SNPs in human neuroblastoma cell line SH-SY5Y, F(4) = 7.87, ***p < 0.001, posthoc analysis: tpGL3-rs2660304 (4.482), ***p < 0.001, r = 0.91; trs2660304-rs2802535 (–4.57), ***p < 0.001, r = 0.92; trs2660304-rs1625579 (–4.57), ***p < 0.001, r = 0.92; tpGL3-rs2660304 (1.65), nsp = 0.4781, r = 0.64, n: three experiments. b. Fold-change differences of MIR137 determined by qRT-PCR from Fig. 1c, separated by its individual lines, WiN= 65, *p = 0.0157, r = –0.40, box plot shows, in ascending order, the lowest maximum value, the first quartile, median, third quartile and the highest maximum value, dots are outlier. c. Summary table of the human fibroblasts and their genotype alleles for the four disease-associated SNPs. First four are homozygous for common major alleles; the last two are homozygous for the minor alleles. d. Examples of successfully induced neurons before FACS. Left: bright field, middle: transduced cells, right: expression of the neuron-specific HSV-CamKII-mCherry construct. Scale bar: 100 μm. Before any iN experiments, cells were visually controlled for successful reprogramming. e. Relative microRNA levels in induced neurons from common major and minor allele SNPs determined by RT-qPCR, tmiR-9(7.87) = –0.2, nsp = 0.8475, r = 0.07, tmiR-19b(8.28) = 0.12, nsp = 0.9047, r = 0.04, tmiR-124(13.89) = –0.16, nsp = 0.8773, r = 0.04, box plot shows, in ascending order, the lowest maximum value, the first quartile, median, third quartile and the highest maximum value, dots are outlier, n: number of samples from at least two reprogramming, s.e.m.
