Supplementary Figure 12: qPCR validation of RNA sequencing results.
From: Experience-dependent DNA methylation regulates plasticity in the developing visual cortex
(a) Npas4 gene expression in the visual cortex of MD3d mice and MD3d mice treated with RG108 or vehicle. Monocular deprivation significantly downregulated the expression of Npas4 in the visual cortex contralateral to the deprived eye (cortex ipsilateral to the deprived eye vs. contralateral cortex n = 11, Paired t-test, t = -3.9, P = 0.002; MD3d vehicle vs MD3d RG108, n = 8, Student t-test, t = 4.43, P < 0.001). (b) Gadd45g decrease in the visual cortex contralateral to the deprived eye (data shown in Suppl. Fig. 3) was counteracted by RG108 infusion during the monocular deprivation (MD3d vehicle vs. MD3d RG108, n = 6, Student t-test, t = 2.48, P = 0.04). (c) qPCR in the visual cortex of MD3d critical period mice showed a significant downregulation of Nr4a1 in the contra ctx with respect to the ipsi ctx. (n=6, ipsi ctx vs contra ctx, paired t-test, t=3.07, P=0.02). The effect on Nr4a1 expression is completely reversed by RG108 treatment during MD3d (n=3, vehicle vs RG108, paired t-test, t=3.67, P=0.02). Error bars represent SEM.
