Supplementary Figure 1: Monocular deprivation regulates DNMT expression in the visual cortex of critical period mice.
From: Experience-dependent DNA methylation regulates plasticity in the developing visual cortex
(a) MD3d significantly increased the expression of Dnmt3a, Dnmt3b and Dnmt1 mRNA in the visual cortex contralateral (contra ctx) to the deprived eye with respect to the ipsilateral cortex (ipsi ctx) (cortex ipsilateral to the deprived eye vs. contralateral cortex, signed rank test, Dnmt3a n = 18, W = 5, P = 0.02; Dnmt3b n = 13, W = 79, P = 0.003; Dnmt1 n = 13, W = 89, p < 0.001). (b) Dnmt3a, Dnmt3b and Dnmt1 expression in the right (right ctx) and left cortex (left ctx) of aged matched non deprived (Nondep) animals. No significant differences were found (right ctx vs. left ctx, n = 10, signed rank test, Dnmt3a W = 5 P = 0.82; Dnmt3b W = 23, P = 0.92; Dnmt1 W = 34,P =0.21). (c) qPCR quantitation of Dnmt3a (a), Dnmt3b (b) and Dnmt1 (c) in the visual cortex of MD3d and age matched non deprived mice are expressed as percentage of Gapdh. (MD3d: ipsi ctx vs. contra ctx, Dnmt3a n = 18, W = 97; Dnmt3b n=13,W=-79;Dnmt1n=13,W=89,Signed rank test p<0.05. Nondep: n=10, Dnmt3aW= 5, P = 0.81; Dnmt3b W = -3, P = 0.92; Dnmt1 W = 34, P = 0.2) (d) Western blot analysis of DNMT3a and DNMT1 in the visual cortex of MD3d mice (DNMT3A n = 13, W = 72; DNMT1 n = 16, W = 120, signed rank test P < 0.001). Error bars represent SEM.
