Supplementary Figure 4: Specificity of the MeDIP experiment.
From: Experience-dependent DNA methylation regulates plasticity in the developing visual cortex
(a) Sketch of miR212/132 and Bdnfex-4 CRE sites and interrogated sequences. (b) Normal rabbit IgG caused a statistically significant less recovery of methylated DNA with respect to a specific antibody against 5-mC. BDNFex-4b, BDNFex-4c and CRE2-3 sequence abundance was measured using qPCR in cortical samples immunoprecipitated with antibodies specific for 5-mC or Normal rabbit IgG. Data were normalized to the average recovery obtained with the antibody for 5-mC. (BDNFex-4b: n = 11, T = 72; BDNFex-4c T = 92, n =12; CRE2-3 n = 11, T = 75, 5-mC vs Normal rabbit IgG, Mann-Withney rank sum test p<0.001. (c) 100% methylated DNA and DNA from visual cortical samples were immunoprecipitated with purified antibody against 5-mC. 100% methylated DNA shows a significantly higher recovery in respect to the other sample. (n = 5, T = 16, Mann-Withney rank sum test P = 0.005). Error bars represent SEM.
