Supplementary Figure 8: Determination of intestinal bacterial loads and complexity.
From: Host microbiota constantly control maturation and function of microglia in the CNS
(a) qPCR analysis for eubacterial 16S rRNA could not detect significant amounts of bacteria in the caeca of GF and antibiotic-treated SPF animals. Representative data from fifteen SPF, five GF, six SPF (ABX), five ASF and five ASF (recol.) mice are depicted. Gene copy numbers were normalized to DNA content. Each symbol represents one mouse. Means ± s.e.m. are indicated. Significant differences were determined by an unpaired t test and marked with asterisks (*P < 0.05, **P < 0.01). P values: SPF vs. GF: 0.0151, SPF vs. SPF (ABX): 0.0046, SPF vs. ASF (recol.): 0.0280.
(b) Recolonized ASF mice (ASF recol.) harbour a greater microbial diversity as detected by qPCR using group specific primers. Besides Parabacteroides (ASF357), Lactobacillus (ASF 361) and Clostridium (ASF356), mouse intenstinal Bacteroides (mib) and Enterobacteriaceae groups are detectable in the caecum of SPF and re-colonised ASF animals using group-specific primers, respectively.
