Supplementary Figure 6: Characterization of excitatory synaptic transmission in Nrxn3 mutant olfactory bulb neurons.
a-c, Quantification of immunocytochemistry of Nrxn3α/β cKO olfactory bulb cultures infected with lentiviruses expressing inactive (control) or active (cre) cre-recombinase fused to EGFP. Puncta density (b; gephyrin: p = 0.884; vGAT: p = 0.176; vGluT1: p = 0.465), size (c; gephyrin: p = 0.262; vGAT: p = 0.170; vGluT1: p = 0.645) and intensity (d; gephyrin: p = 0.737; vGAT: p = 0.851; vGluT1: p = 0.693) representing gephyrin, vGAT and vGluT1 immunoreactivity.
d, Summary graphs of the passive membrane properties measured in mitral/tufted cells in Nrxn3α/β cKO olfactory bulb cultures infected with lentiviruses expressing inactive (control) or active (cre) cre-recombinase fused to EGFP (input resistance: p = 0.728; capacitance: p = 0.583).
e, Sample traces (left) and summary graphs representing mEPSC kinetics measured in Nrxn3α/β cKO olfactory bulb cultures infected with lentiviruses expressing inactive (control) or active (cre) cre-recombinase fused to EGFP (mEPSC rise: p = 0.816; decay: p = 0.286).
f, Same as in (d) but passive membrane properties measured in mitral/tufted cells from Nrxn3SS4+ KI olfactory bulb cultures infected with lentiviruses expressing inactive (Nrxn3SS4+) or active (Nrxn3SS4–) cre-recombinase fused to EGFP (input resistance: p = 0.921; capacitance: 0.894).
g, mEPSC kinetics from Nrxn3SS4+ or Nrxn3SS4– primary olfactory bulb cultures (mEPSC rise: p = 0.626; decay: p = 0.693).
Data shown represent means ± SEM calculated from total number of experiments. Numbers in bar graphs represent number of cells/independent experiments. Statistical significance was assessed by single-factor ANOVA.
