Supplementary Figure 7: Properties of mIPSCs in cultured olfactory bulb neurons from the three different types Nrxn3 mutant animals.
a. mIPSC cumulative probability plots for frequency (left) and amplitude (right) from Nrxn3α/β cKO mitral/tufted neurons in (p < 0.0001).
b, Representative traces (left) and summary graphs (right) of mIPSC decay and rise kinetics from Nrxn3α/β cKO mitral/tufted neurons in (mIPSC rise: p = 0.462; decay: p = 0.991).
c, Representative traces (left) and summary graph (right) measuring the sucrose-evoked readily releasable pool (RRP) of GABAergic vesicles in Nrxn3α/β cKO olfactory bulb cultures (p = 0.303).
d & f, mIPSC cumulative probability plots as in (a) but from cultured Nrxn3α KO mitral/tufted neurons (d; p < 0.0001) or from cultured Nrxn3 SS#4 KI neurons infected with lentiviruses expressing inactive Cre (Nrxn3SS4+) or active Cre (Nrxn3SS4-) recombinase (f; p = 0.671).
e & g, mIPSC kinetics as in (b) but in Nrxn3α KO mitral/tufted neurons (e; mIPSC rise: p = 0.939; decay: p = 0.943) and Nrxn3SS#4 KI (g; mIPSC rise: p = 0.520; decay: p = 0.713).
h & i, Cartoon schematic of full-length (h) and GPI-anchored (i) Nrxn3α rescue constructs used in Figs. 7d and 7e. Note: GPI-Nrxn3α is incapable of activating intracellular signaling.
Data represent means ± SEM calculated from independent experiments. Numbers in bar graphs represent number of cells/independent experiments. Statistical significance in b, c, e, g was assessed by single-factor ANOVA. Statistical significance for cumulative probability plots was determined by K-S test.
