Supplementary Figure 8: Nerve injury-induced microglia responses are present in microglial-specific Bdnf mutant mice of both sexes.
From: Different immune cells mediate mechanical pain hypersensitivity in male and female mice
a) Spinal cord dorsal horn microgliosis following SNI in Cx3cr1CreER x loxP‑Bdnf mice. Insets are high power images taken from the ipsilateral dorsal horn of the spinal cords from female (left) and male (right) mice. Microglia were labelled with Iba1 (red). Both males and females show the characteristic microglial proliferation around the central terminals of peripherally axotomised sciatic afferents. b) Microglial-specific Bdnf mutant express a YFP IRES element. YFP (green) colocalizes directly with microglia (Iba1; red) in the spinal cord. c) Other cellular populations are identical in male and female microglial-specific Bdnf mutant mice after SNI. Astrocytes and neuronal nuclei are shown by GFAP and NeuN immunohistochemistry (both blue), respectively.
