Supplementary Figure 13: Adoptive transfer of splenocytes into female Rag1−/− mice reinstates their use of the female glial-independent pathway.
From: Different immune cells mediate mechanical pain hypersensitivity in male and female mice
a) Successful repopulation by adoptive transfer. Spleen sections taken from a female Rag1−/− mouse (left) and a female Rag1−/− mouse following adoptive splenocyte transfer (right). Spleen macrophages are labelled in green (Iba1) and repopulated T-cells in red (CD3). Scale bar = 200 μm. b) Adoptive transfer of splenocytes (Splen.) from immunocompetent female (F) Rag1+/+ into immunocompromised female Rag1−/− mice (i.e., Rag1−/− F + Splen. condition) restores the male (M)-like ability of i.t. MCL (50 μg; 3 days after injection; D3) to reverse CFA allodynia. Rag1−/− F + Veh. indicates mutant females which received the adoptive transfer vehicle without splenocytes. Symbols represent mean ± SEM 50% withdrawal threshold from von Frey filaments before CFA (BL), 3 days after CFA, pre-MCL injection (D3), and 10–120 min post‑injection of MCL (n=4–6 mice/sex/condition except for adoptive transfer group, n=10). Repeated measures ANOVA revealed a significant group x repeated measures interaction: F15,100 = 5.8, p<0.001. ***p<0.001 compared to Rag1+/+ F and Rag1−/− F + Splen. groups by Tukey’s posthoc test.
