Supplementary Figure 6: Microglial depletion by intrathecal Mac-1-saporin treatment in male and female mice. | Nature Neuroscience

Supplementary Figure 6: Microglial depletion by intrathecal Mac-1-saporin treatment in male and female mice.

From: Different immune cells mediate mechanical pain hypersensitivity in male and female mice

Supplementary Figure 6

Depletion of microglia ipsilateral (Ipsi.) to the SNI (7 days post‑surgery) in both male (M) and female (F) mice 4 h after treatment with Mac‑1‑saporin (Mac-1-SAP) toxin compared to mice treated with saporin (SAP) vehicle. Bars represent mean ± SEM Iba1‑positive (Iba1+) cells in the lumbar spinal cord dorsal horn (n=5 mice/sex/condition). Cont.=contralateral. Similar microglial depletion (≍25%) was observed in both sexes (male: t8 = 2.4, p=0.02; female: t8 =2.8, p=0.001). *p<0.05.

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