Supplementary Figure 3: Quantification of total DAT expression in synaptosomal preparations derived from Vav2–/– mice and WT control under various experimental conditions.
From: Identification of a Vav2-dependent mechanism for GDNF/Ret control of mesolimbic DAT trafficking
a-c, correspond to Fig. 2f-i. (a, n = 4). Two-way ANOVA with Bonferroni's multiple comparisons test. F(1, 12) = 1.067, P > 0.9999 for Nac, P = 0.6297 for dSTR. (b, c, n = 3). One-way ANOVA with Bonferroni's multiple comparisons test. b, F(2, 6) = 0.2230, P = 0.7976 between control and shVav2 #1, P = 0.8972 between control and rescue. c, F(2, 6) = 0.1670, P > 0.9999 between control and RFP-Vav2, P > 0.9999 between control and Y172F. d, corresponds to Fig. 3b. (n = 3). Two-way ANOVA with Bonferroni's multiple comparisons test. F(1, 8) = 0.1616. P > 0.9999 between NAc WT and NAc Ret+/−, P > 0.9999 between dSTR WT and dSTR Ret+/−. e, f, correspond to Fig. 4e, g. (n = 3). e, unpaired two-tailed t-test. (f, n = 2). Two-way ANOVA with Bonferroni's multiple comparisons test. F(1, 4) = 0.2292, P > 0.9999 between scrambled control and scrambled GDNF, P > 0.9999 between shVav2 control and shVav2 GDNF. g, h, correspond to Fig. 6b, c. (g, n = 2). Two-way ANOVA with Bonferroni's multiple comparisons test. F(1, 4) = 0.5856, P > 0.9999 between WT saline and WT cocaine, P> 0.9999 between Vav2 mutant saline and Vav2 mutant cocaine. (h, n = 4). Two-way ANOVA with Sidak's multiple comparisons test. F(1, 12) = 0.3031. P = 0.9998 between WT saline and WT cocaine, P = 0.9983 between Vav2 mutant saline and Vav2 mutant cocaine. n.s. no significance. The data are expressed as ratios of total DAT to β-actin. Error bars represent s.e.m.
