Supplementary Figure 6: Involvement of PKC in the Vav2-mediated DAT activity.
From: Identification of a Vav2-dependent mechanism for GDNF/Ret control of mesolimbic DAT trafficking
a, shVav2 #2-induced Vav2 knockdown attenuates PMA-induced reduction of DAT activity in N2a cells stably expressing RFP-DAT and GFRa1. Two-way ANOVA with Bonferroni‘s post-hoc test was performed. (n = 10). Main effect of genotype (F(1, 36) = 38.06, P <0.0001), treatment (F(1, 36) = 58.23, P <0.0001) and interaction (F(1, 36) = 4.376, P = 0.0436); post hoc: t(36) = 6.875, P < 0.0001 for Scrambled group, t(36) = 3.917, P = 0.0008 for shVav2 group. *P < 0.05. b, Treatment of N2a cells stably expressing RFP-DAT and GFRa1 with either PMA (1 mm, 30 min) or GDNF (100 ng/ml, 30 min) significantly down-regulate DAT-mediated DA uptake. These effects could not be significantly attenuated by pretreatment with PKC inhibitor GF-109203X (1 mM, 30 min). One way ANOVA with Tukey's multiple comparisons test was performed. (n = 11). F(3, 40) = 28.13, P<0.0001; q(40)=12.58, P < 0.0001 between control and PMA, q(40) = 8.185, P < 0.0001 between Control and GDNF, q(40) = 3.219, P = 0.1208 between GDNF and GDNF+GF. *P <0.05. Error bars represent s.e.m.
