Supplementary Figure 7: Characterization of interaction among Vav2, DAT and Ret.
From: Identification of a Vav2-dependent mechanism for GDNF/Ret control of mesolimbic DAT trafficking
a, Colony growth assay on the SD-His medium showed the DAT-Cub-LexA-VP16 and Ret-NubG were expressed with no self-activation of the histidine reporter gene expression. b, Co-IP assays show that treatment with PP2 does not alter their interaction between Ret and DAT in HEK293 cells. c, Immunoblotting reveals that activation of Ret signaling by overexpression of Ret-Flag in HEK293 cells does not cause significant alteration in the levels of phosphor-Thr53-DAT. These experiments were repeated at least twice. c shows cropped blot images from their corresponding full-length blots presented in Supplementary Figure 12.
