Supplementary Figure 8: LSD1n is required for learning and memory
From: LSD1n is an H4K20 demethylase regulating memory formation via transcriptional elongation control
a) Analysis of brain anatomy of WT and LSD1n KO mice using Nissl stain, revealing normal brain morphology of LSD1n KO mice;
b) Optomotor behavioral test of male or female WT and LSD1n KO mice revealing normal vision in LSD1n KO mice. Data are shown as mean ± SD; N.S. = non statistically significant (p=0.111), ANOVA test (n=10);
c) Percentage of spontaneous alternation (Y maze) showing normal working memory in LSD1n KO mice. Data are shown as mean ± SD; N.S. = non statistically significant (p=0.7449); ANOVA test (n=10);
d) Number of total arm entries (Y maze) showing normal working memory in LSD1n KO mice. Data are shown as mean ± SD; N.S. = non statistically significant (p=0.0550); ANOVA test (n=10);
e) Normal locomotor activity in LSD1n KO mice assessed by ambulation counts in dark and light cycles (n=10);
f) Western blot analysis showing increased H4K20 methylation in cortex of LSD1n knockout mice. The amount of protein is quantified using ImageJ software, the first control lane has an arbitrarily assigned value of 100;
g) RT-PCR analysis showing decreased expression of LSD1n in 28-month-old mice (Old) compared to 4-month-old mice (Young);
h) RT-qPCR showing decreased expression of LSD1n in 28-month-old mice (Old) compared to 4-month-old mice (Young). Data are shown as mean ± SD; N.S. = non statistically significant, ** = P-value<0.01 (p=0.1896 LSD1; p=0.0001 LSD1c; p=3.7E-5 LSD1n; n=3 technical replicates from young or old mice; unpaired t-test).
