Supplementary Figure 3: Both LSD1n and LSD1c interact with histone H3 and H4 tails
From: LSD1n is an H4K20 demethylase regulating memory formation via transcriptional elongation control
a) Histone peptide pull-down assay using histone H3 and H4 peptides and recombinant LSD1c protein revealing binding between LSD1c and histone H3 or H4 by Western blot using anti-His probe. * Truncated form of His-LSD1c;
b) Histone peptide pull-down assay using histone H3 and H4 peptides and recombinant LSD1n protein, revealing binding between LSD1n and histone H3 or H4 by Western blot using anti-His probe. * Truncated form of His-LSD1n;
c) Histone peptide pull-down assay using histone H3 and H4 peptides and recombinant CoREST protein, revealing specific binding between CoREST and H4 (1-23) by Western blot using anti-His probe;
d) Coomassie staining and Western blot using anti-GST or anti-His probe antibodies, showing expression of the recombinant GST-CoREST-ELM2-His protein;
e) MODified histone peptide array using GST-CoREST-ELM2-His recombinant protein revealing specific interaction between ELM2 domain of CoREST and histone H4 tails by Western blot using anti-GST antibody.
