Supplementary Figure 7: Delivery of CRE-DOG^OPT to the mouse nervous system by rAAV.

(a) Co-injection of rAAV (serotype 2/8) encoding EF1α-driven N-CretrcintG and C-CreintG and rAAV-FLEX-tdT into the P0 murine retina along with either rAAV encoding EF1α-driven GFP (top row) or ZsGreen (bottom row). Retinas were harvested between P21–30. Top row: GFP-dependent activation of tdT in the horizontal cell layer of the retina. Bottom row: little background CRE-DOG^OPT activity was observed. Note: ZsGreen was found to aggregate heavily in the retina. Images representative of at least 3 retinas per condition. Scale bar, 30 μm. (b) Little to no background FLEX-tdT activity in rAAV infected cells of the retinal GCL. Co-injection of rAAV (serotype 2/8) encoding EF1α-driven N-CretrcintG and C-CreintG and rAAV-FLEX-tdT into the murine retina. Mice were of age P77 at time of injection. Retinas were harvested 3 weeks post-infection and immunostained for polyclonal Cre antibody (left panel). No tdT expression was detected in Cre-immunopositive cells of the ganglion cell layer (GCL) (center panel). Merge of all channels with DAPI (right panel) suggests that faint red dots were background signals or debris and were not cells. Images are representative of 3 retinas. Scale bar, 20 μm.