Supplementary Figure 3: Comparative observation of optical transparency and fluorescence preservation between cleared and PBS(–)-treated hemispheres and 2-mm-thick brain slices | Nature Neuroscience

Supplementary Figure 3: Comparative observation of optical transparency and fluorescence preservation between cleared and PBS(–)-treated hemispheres and 2-mm-thick brain slices

From: ScaleS: an optical clearing palette for biological imaging

Supplementary Figure 3

(a) ScaleS, CUBIC, 3DISCO, and SeeDB were evaluated compared with PBS(–). Images (except for #1) comparing the clarification (T: transmission) and preservation of YFP fluorescence (FL) between cleared (left) and PBS(–)-treated (right) hemispheres from YFP-H (solid bar) and ChR2-YFP (open bar) mice are shown. The age in week (W) and the gender of each mouse is shown in parentheses. Transmission images were taken using a single-lens reflex camera (NIKON D7000) with a patterned background. Fluorescence images were taken using a fluorescence stereomicroscope (NIKON SMZ18) with a color CCD camera (ZEISS AxioCam 506 color).

(b) ScaleS and PACT were evaluated compared with PBS(–). Images (except for #1) comparing T and FL between cleared (left) and PBS(–)-treated (right) brain slices (2 mm thick) from YFP-H mice are shown. Image acquisition was performed in the same way as described in (a).

Scale bars: 5 mm. See Fig. 2 for the quantitative measurements and the representative images (#1).

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