Supplementary Figure 4: Clearing young mouse samples by ScaleS and SeeDB
From: ScaleS: an optical clearing palette for biological imaging
Two mouse embryos (E13.5) and the left and right halves of the brain of a neonatal mouse (P3) were used. After fixation with 4% PFA, they were cleared according to the protocols of ScaleS (Supplementary Fig. 1b) and SeeDB (Ref. 5). At time points indicated, clarification of the samples was examined by taking photos. The immersion solutions at picture acquisition are shown (right). Scale bar: 5 mm.
