Supplementary Figure 8: Volume preservation after AbScale and ChemScale treatment
From: ScaleS: an optical clearing palette for biological imaging
(a) A pair of fixed hemispheres was prepared from a C57BL/6J mouse (12 weeks old); the left hemisphere was AbScaled with Alexa488-A60 (anti-NeuN mAb) and the right one was incubated in PBS(–). Fluorescence (FL) and transmission (T) images were taken using a fluorescence stereomicroscope. Scale bars: 5 mm. The sample volume was measured by liquid displacement. The volume ratio of the stained sample to untreated sample was plotted on a bar graph. The experiment was duplicated.
(b) A pair of fixed hemispheres was prepared from a C57BL/6J mouse (12 weeks old), and the left hemisphere was ChemScaled with propidium iodide (PI) and the right one was incubated in PBS(–). Fluorescence (FL) and transmission (T) images were taken using a fluorescence stereomicroscope. Scale bars: 5 mm. The sample volume was measured by liquid displacement. The volume ratio of the stained sample to untreated sample was plotted on a bar graph. The experiment was duplicated.
