Supplementary Figure 11: Plaque-centered investigation of microglial association in the brain of aged AppNL-F/NL-F mice at different ages
From: ScaleS: an optical clearing palette for biological imaging
Each histogram shows the distribution of the distances of activated and resting microglia to the plaque edge for individual plaques. Isolated plaques that were not associated with any activated microglia in their vicinity (≤ 21 μm) are labeled in blue. Other plaques showing microglial association in their vicinity (≤ 21 μm) are labeled in red. Vertical dotted lines are drawn at 21 μm to distinguish between closely neighboring and distant microglia.
(a) Thirty-nine plaques in the brain region of a 20-month-old AppNL-F/NL-F mouse (see Fig. 6a–h).
(b) Twenty-seven plaques in the brain region of a 10-month-old AppNL-F/NL-F mouse.
(c) Sixteen plaques in the brain region of a 12-month-old AppNL-F/NL-F mouse.
(d) Thirty-one plaques in the brain region of a 14-month-old AppNL-F/NL-F mouse.
(b–d) Two volume rendered images (xy) are shown on the right. top, a low-magnification image generated from the observed region; bottom, a high-magnification image containing analyzed plaques.
(e) A bar chart showing the percentage of isolated plaques analyzed at age 10, 12, 14, and 20 months.
Scale bars in the perspective VR images (yellow) show the distance on the foreground plane: 50 μm.
