Supplementary Figure 5: 5HT1AR expression in abGCs.
From: 5-HT1A receptors on mature dentate gyrus granule cells are critical for the antidepressant response
a) Construction of Nestin-CreER/fl1A mice and timeline of experiment in b. Floxed 1A mice were generated and crossed with Nestin-CreERT2 mice. Upon Cre-mediated excision, a yellow fluorescent protein (YPet) was expressed under control of the 5HT1AR promoter and marked the cells where 5HT1AR had been deleted. Timeline indicates pretreatment protocol used in experiment shown in b. Nestin-CreER/fl1A mice were gavaged twice per day for three days with 200mg/kg tamoxifen when they were 54, 55, and 56 days old. Mice were then sacrificed 2 days, 1 week (9 weeks old), 2 weeks (10 weeks old), 3 weeks (11 weeks old), and 4 weeks (12 weeks old) after the final tamoxifen administration. n = 6 per time point. b) Representative sections from Nestin-CreER/fl1A mice treated with tamoxifen when they were 8 weeks old and then sacrificed 3 weeks (11 weeks old; left) or 4 weeks later (12 weeks old, right). The hilus and dentate gyrus granule cell layer (GCL) are indicated. Scale bar = 60 μm.
