Supplementary Figure 2: NSF control experiments for Figures 1–4.
From: 5-HT1A receptors on mature dentate gyrus granule cells are critical for the antidepressant response
a) NSF controls for POMC-Cre/fl1A and Control mice in Figure 1. b) Percentage weight loss and home cage consumption controls for the NSF experiment. The percentage of weight lost during the deprivation period and the amount of food consumed over 5 min when the mice were placed back into their home cage immediately after NSF exposure were calculated. No significant differences were seen among genotype and treatment groups (Two-Way ANOVA). c) NSF controls for fl1A mice injected with AAV8-CamKII-GFP or AAV8-CamKII-Cre in Figure 2. d) Percentage weight loss and home cage consumption controls for the NSF experiment. The percentage of weight lost during the deprivation period and the amount of food consumed over 5 min when the mice were placed back into their home cage immediately after NSF exposure were calculated. No significant differences were seen among virus and treatment groups (Two-Way ANOVA). e) NSF controls for Nestin-CreER/fl1A mice in Figure 3. f) Percentage weight loss and home cage consumption controls for the NSF experiment. The percentage of weight lost during the deprivation period and the amount of food consumed over 5 min when the mice were placed back into their home cage immediately after NSF exposure were calculated. No significant differences were seen among genotype and treatment groups (Two-Way ANOVA). g) NSF controls for DG-1A+ and 1A KO mice in Figure 4. h) Percentage weight loss and home cage consumption controls for the Novelty Suppressed Feeding experiment. The percentage of weight lost during the deprivation period and the amount of food consumed over 5 min when the mice were placed back into their home cage immediately after NSF exposure were calculated. No significant differences were seen among genotype and treatment groups (Two-Way ANOVA). Bars and error bars throughout the figure represent mean ± SEM.
