Supplementary Figure 4: Photo-suppression of L4 does not alter whisking behavior
From: A direct translaminar inhibitory circuit tunes cortical output
a, Top: Imaging schematic with a high-speed camera for whisker tracking. Middle: example image and tracked whisker (blue line) from the high-speed imaging stream. Bottom: example traces of whisker movement under control conditions (black) and during photo-suppression of L4. Black dots indicated contacts with the tactile stimulus bar. b, Top: Cumulative distribution plot of whisk amplitudes (p = 0.71, n = 422 whisk cycles in 4 mice, 2-way ANOVA, f(1) = 0.14); whisker set point (p = 0.56, n = 422 whisk cycles in 4 mice, 2-way ANOVA, f(1) = 0.33), whisk frequency (p = 0.47, n = 422 whisk cycles in 4 mice, 2-way ANOVA, f(1) = 0.51), and contact rate (p = 0.38, 2-way ANOVA) under control (black) and during L4 photo-suppression (red) (±95% confidence intervals, dotted lines). All error bars are one standard deviation of the mean.
