Supplementary Figure 5: Photo-suppression of L4 preferentially enhances L5 firing for non-optimal stimuli
From: A direct translaminar inhibitory circuit tunes cortical output
a, Scatter plot comparing OMI for the non-preferred stimulus position vs. the preferred stimulus position (mean OMInon-preferred: 0.35±0.04; mean OMIpreferred: 0.02±0.03; p < 10-6, n = 75 units from 9 mice, paired t-test, t(74) = 8.2). b, Scatter plot of raw firing rates of L5 RS units for the least-preferred stimulus position comparing control and L4 photo-suppression conditions (p < 10-6, n = 75 units from 9 mice, paired t-test, t(74) = 7.9). c, As in b) except for the most-preferred stimulus position (p = 0.0006, n = 75 units from 9 mice, paired t-test, t(74) = 3.7). d, Histograms of a mean OMI of L5 RS units between animals tested will all the whiskers intact (‘full pad’, black), and those trimmed to the principal whisker (green). e, As in d) except for L5 FS units. L4 suppression significantly (p = 1.3×10-4, n = 24, f(1) = 15) enhanced L5 RS cells and significantly (p<0.002, n = 12, f(1) = 10) suppressed L5 FS cells during single whisker stimulation (2-way ANOVA). OMI distributions for L5 RS and L5 FS units were not significantly different between full and single whisker stimulation (p = 0.34 and 0.69, respectively, two-tailed t-test).
