Supplementary Figure 6: The impact of L4 photo-suppression on FS units across layers
From: A direct translaminar inhibitory circuit tunes cortical output
a, Plot of the average normalized spike rates for FS units across L2/3, L5 and L6 between control and L4 photo-suppression conditions, grouped by ranked stimulus response. b, Histograms of the mean OMI for FS units across these layers across all stimulus conditions. c, Plot of the mean OMI across all stimulus conditions for FS units across all layers during photo-suppression of L4. d, As in c) but for L4 photo-activation (n.d., not done). e-f As in c) and d) but showing changes in the raw firing rates of FS units across layers. Firing rates in L2/3 (p = 0.005, n = 50 units from 5 mice, 2-way ANOVA, f(1) = 9.13), L4 (n = 35 units from 6 mice, p < 10-6, 2-way ANOVA, f(1) = 26.6), and L5 (p < 10-5, n = 53 units from 9 mice, 2-way ANOVA, f(1) = 24) were significantly reduced during L4 photo-suppression. However, firing rates in L6 were not significantly affected (p = 0.55, n = 25 from 3 mice, 2-way ANOVA, f(1) = 0.36).
