Supplementary Figure 7: Separation of FS and RS units
From: A direct translaminar inhibitory circuit tunes cortical output
a, Example waveforms for units categorized as FS (green) or RS (black) based on several waveform parameters. b, Scatter plot of amplitude asymmetry vs. trough to 2nd peak for recorded units. Units were categorized by k-means clustering. FS units: green, RS units: black, grey: excluded units, red: cells recorded in cell-attached mode prior to intracellular recording. c, Histogram of sEPSC decay time constants in identified FS (green) or RS neurons (black) from acute brain slices. Cells were identified by their firing characteristics to current injection in the whole-cell mode. Inset: example average sEPSCs from an FS neuron (green) and an RS neuron (black). d, As in c) but for neurons recorded using the blind patch technique in vivo. e, Example supra-threshold response of a PV+ neuron recorded with a K+-based internal solution in a brain slice to current injection showing the characteristic non-adapting high frequency firing. f, As in e) but for a blind-patched pFS L5 neuron in vivo. Note that the variance in spike timing likely arises due to fluctuations in the background synaptic conductance in vivo. g, Average estimated series resistance (left), input resistance (center), and membrane time constant (right) for recorded RS and FS L5 cells in vivo (17 cells, 12 mice) and in vitro (in vitro: n = 42 cells in 36 slices from 17 mice). h, Average responses to a -4 mV voltages step in the RS (left) and pFS (center) neurons recorded in vivo. Right: expansion of the average current relaxation in RS and pFS cells after the end of the voltage step.
