Supplementary Figure 9: PV interneuron mediated inhibitory input to L5 cells

a, Average of normalized inhibitory input maps for all L5 pyramidal cells (n = 16 cells in 9 slices from 4 mice) recorded in PV-Cre;flexed-ChR2 brain slices. Maps are vertically aligned to laminar borders and horizontally aligned to the soma of the recorded cell (blue circles). b, Plot of the mean proportion of inhibitory input onto L5 pyramidal cells from PV neurons in each cortical layer, separated by barrel column. H: home column. ±1: adjacent barrel column. Dark gray: mean proportion of home column inhibitory input; light gray: same, but for the surrounding two columns. c, Top: Average spike probability map for all recorded PV neurons (n = 19 ChR2+ PV cells in total, in 11 slices from 5 mice; n = 4 in L2/3; n = 6 in L4; n = 7 in L5; n=2 in L6) under the photo-stimulation conditions using for mapping inhibitory input to L5 PCs. Bottom: Plot of firing probability versus distance from the soma of the recorded PV neuron. d, As in c) except for mean number of action potentials evoked in PV neurons during each photo-stimulus. e, Top: All 16 individual inhibitory input maps for L5 pyramidal cells. Bottom: four example inhibitory input maps in intracellularly recorded PV neurons (recorded at the reversal potential of the ChR2-photocurrent).