Supplementary Figure 10: Impact of photo-suppression of L2/3 excitatory neurons on L5 FS units

a, Example image from a Drd3-Cre mouse injected with a DIO-eNpHR3.0-YFP (green) and recorded with a DiI-labeled laminar multi-electrode array. Scale bar: 200 μm b, Box and whisker plot of the average percent change in L5 FS unit firing rate when photo-suppressing L4 (in the scnn1-tg3-Cre line) or L2/3 (in the Drd3-Cre line). c, Spatial tuning curve of an example photo-suppressed L2/3 RS unit with (red) and without (black) light. d, Raster plot (top) and PSTH (bottom) of the example unit in c) during photo-suppression of L2/3 during sensory stimulation. e, Plot of the proportion of synaptic excitation and synaptic inhibition blocked by application of the glutamatergic antagonists NBQX and CPP (p = 0.00024, n = 13 cells in 8 slices from 4 mice, Wilcoxon signed rank test, z = 3.16). f, Example traces of light evoked IPSCs recorded in a L5 pyramidal cells in drd3-cre mice injected with flexed-ChR2. Black: control, grey: after application of glutamatergic antagonists, showing near 100% block.