Supplementary Figure 1: Data related to figure 1: Selective interaction between ephrin-B3 and PSD-95 in synaptosomes and in vitro.
From: Anchoring and synaptic stability of PSD-95 is driven by ephrin-B3
(a) Source of western blot data shown in Figure 1d (a, top right blot), 1e (a, top left blot), and 1f (a, bottom two blots). Blots show that ephrin-B3 and PSD-95 fail to co-IP from wild type (WT) and ephrin-B3 null (Efnb3–/–) whole brain lysates. Boxes indicate approximate regions show in the main figure. Probed as shown at the bottom of each row of blots.
(b) Source of western blot data shown in Figure 1g (b, top right blot), 1h (b, top left blot), and 1i (b, bottom two blots). Blots show ephrin-B3 and PSD-95 co-IP from P21 wild type (WT) but not ephrin-B3 null (Efnb3–/–) synaptosomes. Boxes indicate approximate regions show in the main figure.
(c) Source of western blot data shown in Figure 1j (c, top left blot), 1k (c, four top right blots), and 1l (c, bottom blots). Blots demonstrate specific co-IP of PSD-95 with ephrin-B3, but not ephrin-B1 or ephrin-B2 from P21 synaptosomes. Boxes indicate approximate regions show in the main figure.
(d) PSD-95-GST and full-length HA-tagged ephrin-B1-B3 were used for the pull-down experiments. Considerably higher pull-down signal was detected with HA-tagged ephrin-B3 than with HA-ephrin-B1 or HA-ephrin-B2. Middle blot shows the source of western loading control blot of HA-tagged ephrin-B proteins used in the binding assay.
(e) Source of western blot data shown in the pull-down (top blot panel S1d). Boxes indicate approximate regions show in the figure.
(f) Source of western blot data shown the flow-through (bottom blot panel S1d). Boxes indicate approximate regions show in the main figure.
