Supplementary Figure 3: IgSF11 interacts with PSD-95 in yeast two-hybrid assays.
From: Synaptic adhesion molecule IgSF11 regulates synaptic transmission and plasticity
The C-terminal PDZ-binding motif of IgSF11 interacts with PDZ domains of PSD-95 family proteins (PSD-95, PSD-93/chapsyn-110 and SAP97) in yeast two-hybrid assays. pBHA, bait vector; pGAD10, prey vector; WT, wild type (last seven residues); VA, a mutant IgSF11 in which the last residue (valine) was changed to alanine. PDZ domains from GRIP2, Shank1 and NHERF were used as controls. β-Galactosidase (β-gal) activity: +++, < 45 min; ++, 45–90 min; +, 90–240 min; –, no significant β-gal activity. Three independent experiments were performed.
