Supplementary Figure 4: IgSF11 does not induce clustering of pre- or postsynaptic proteins in contacting axons and dendrites, and does not interact with other synaptic adhesion molecules.

(a-c) Cultured rat hippocampal neurons were cocultured with HEK293T cells expressing rat IgSF11 (untagged), or EGFP (control; DIV 10–13), followed by staining for synapsin I (a presynaptic protein), PSD-95 (an excitatory postsynaptic protein), and gephyrin (an inhibitory postsynaptic protein). Scale bar, 30 μm. (d) IgSF11 does not interact with other synaptic adhesion molecules, as shown by the lack of binding of the ectodomain of IgSF11 fused to human Fc (IgSF11-ecto-Fc) to known synaptic adhesion molecules expressed in HEK293T cells. IgSF11-expressing HEK293T cells were used as a control. Scale bar, 10 μm. Three independent experiments were performed.