Supplementary Figure 10: Dopamine release on No-Go trials in experiment 2 reflects correct action initiation.

(a, b) Average dopamine release (mean ± S.E.M.) on correct No-Go trials (green lines) compared to incorrect No-Go trials when the animals left the nose-poke prematurely (blue lines). Data is aligned to cue onset (a) or nose-poke exit (b). Large Reward trials are plotted with filled lines, Small Reward with dashed lines. Owing to the smaller proportion of Go trials in each session and increased success rate on Go Large trials, there were too few Incorrect Go trials to analyse in experiment 2 (< 2%). (c, d) Average No-Go dopamine release (mean ± SEM) on a subset of correctly performed No-Go trials where the rats’ exit from the nose-poke was either “fast” (< 600 ms after cue offset, filled green line) or “slow” (> 1,600 ms after cue offset, dashed green line). Data is aligned to cue offset (which occurred 1 s before reward delivery in experiment 2) (c) or nose-poke exit (d). Therefore, the fast head exit times occur > 400 ms before reward delivery and the slow ones > 600 ms after reward delivery.