Supplementary Figure 6: Dopamine on failed No-Go trials decreases when the error is signaled.

Average unsmoothed dopamine signals from experiment 1 (mean ± S.E.M.) recorded during successful (filled line) and failed (dotted line) No-Go trials (trials where the animal exited the nose poke before the end of the No-Go hold period) aligned to time of (a) cue onset or (b) head exit from nose-poke (when the error is signaled). Lower panels show average discriminability between the Correct and Failed No-Go trials for each timepoint (shaded area = population of 1000 permuted sessions; line: *, p < 0.05 permutation tests, corrected for multiple comparisons). (c) Boxplot of the average coefficients for the slopes of the linear fit of the dopamine signal in a 2.5 s window following cue onset for correct and failed No-Go trials (central mark is the median, the edges of the box are the 25^th and 75^th percentiles, the whiskers extend to the most extreme data points). The decrease in DA following cue onset is significantly greater in failed no-go trials than in successful no-go trials (*, p = 0.02, W₇ = 0, Wilcoxon Signed Rank Test).