Supplementary Figure 5: Trafficking parameters of protective APP(Ice) mutant.
From: Visualizing APP and BACE-1 approximation in neurons yields insight into the amyloidogenic pathway
(a) Construct design and experimental strategy to evaluate internalization of APP.
(b) Similar internalization of WT and APP(Ice) mutants in neurons, quantified on right (26-28 neurons from three independent experiments were analyzed).
(c) Comparing vesicle transport kinetics of APP WT and APP(Ice) in dendrites. Neurons were transfected with APP:GFP or APP(Ice):GFP, and were imaged live with fast image acquisition (1 frame/sec for 200 secs), 5-6 h post-transfection. Note that various transport parameters – frequency, velocity and run length – are largely similar between the two groups, though a slight decrease in anterograde particle frequency was seen with APP(Ice) mutant. ~ 450 particles from 28-36 neurons – from three separate experiments – were analyzed; * p < 0.05.
