Supplementary Figure 7: Neuronal recycling endosomes are acidic.
From: Visualizing APP and BACE-1 approximation in neurons yields insight into the amyloidogenic pathway
Neurons were co-transfected with TfR:pHluorin and soluble mCherry for ~5-6 h and imaged live. TfR:pHluorin is the recycling endosome marker (TfR) tagged to a pHsensitive probe (pHluorin). Note that the TfR:pHluorin fluorescence increases upon incubation in an alkaline media – indicating that these vesicles are acidic (middle panel) – and quenches in an acidic media (bottom panel), with little change in the soluble mCherry fluorescence (right panels).
