Supplementary Figure 2: Tamoxifen-independent recombination in oligodendrocytes of the reporter mice.
From: Oligodendrocyte death results in immune-mediated CNS demyelination
(a) Expression of the reporter gene protein YFP (green) was detected in a subpopulation of oligodendrocytes stained for CC-1 (red) in all CNS areas examined of the untreated PLP/CreERT;RosaEYFP (reporter w/o 4-HT; lower panel) mice at the age of 52 weeks. CC1/YFP stainings in corresponding areas of control littermate mice (RosaEYFP) are shown in the upper panel. The CC1/YFP double positive cells (yellow) are marked by arrows. Images are representative of three mice/genotype. Scale bar: 100 μm. (b) Counts of the CC1/YFP double positive cells labeled in different CNS areas of the untreated reporter mice showed that tamoxifen-independent recombination occurs in ~ 6%-14% of oligodendrocytes at 26 weeks and it tends to be increased in most areas (6%-20%) at 52 weeks (brain stem p =0.0084). Graph in b shows means (+ SEM), n=4 mice per group, two-tailed unpaired Student’s t test, **p <0.01.
