Supplementary Figure 7: Adoptive transfer of MOG-specific T cells derived from tamoxifen-treated DTA mice causes mild demyelination in naive Rag1−/− mice.
From: Oligodendrocyte death results in immune-mediated CNS demyelination
Splenic cells from tamoxifen-treated (PLP/CreERT;ROSA26-eGFP-DTA) DTA mice at 40-52 weeks post-injection or age-matched control (ROSA26-eGFP-DTA) mice were cultured for 72 h at in the presence of MOG35–55 peptide (20 μg/ml). Cultured cells from tamoxifen-treated DTA mice (2x106 blast cells) were transferred into naïve Rag1−/− recipient mice (a) Forty-two days after transfer, Electron microscopy analysis revealed foci of myelin loss in the lower lumbar spinal cord and cerebellar white matter in Rag1−/− mice inoculated with T cells from tamoxifen-treated DTA mice (DTA recipient) as compared with those inoculated with cells from control mice (littermate recipient). Inflammatory cells were also frequently detected in the cerebellum (asterisk). More-extensive demyelination was seen in the brain stem gray matter. Images are representative of three mice/genotype. Scale bars: 2 μm. (b) Demyelination in the brain stem of Rag1−/− mice inoculated with cells from tamoxifen-treated DTA mice was shown by significantly reduced mean (+ SEM) numbers of myelinated axons (~30% fewer, p=0.0363). N=3 mice/group; *p <0.05 with two-tailed unpaired Student’s t test.
