Supplementary Figure 2: Thalamic axons project to L1 through L4 of mouse V1.
From: Thalamus provides layer 4 of primary visual cortex with orientation- and direction-tuned inputs
(a) GFP labels visual thalamic neurons and tdTomato labels L4 neurons in Scnn1a-Tg3-Cre mice. (b) GFP+ neurons in dLGN. d, dorsal; m, medial. Scale bar: 100 μm. (c) GFP+ thalamic axons and tdTomato+ L4 neurons in a V1 section from pia (top) to white matter (bottom). Scale bar: 50 μm. (d) Enlarged views of GFP+ axons of areas i–iv in c. Scale bar: 10 μm. (e) In widetype mice with GCaMP6s+ thalamic axons, the same projection pattern was observed from pia (top) to white matter (bottom). Scale bars: 50 μm. (f) Enlarged views of GCaMP6s+ axons of areas i–iv in e. Scale bar: 10 μm. We found no GFP+ or GCaMP6s+ cell bodies in V1. Representative images from 3 mice (GFP and tdTomato) and 21 mice (GCaMP6s).
