Supplementary Figure 7: Fine-scale retinotopic organization.
From: Thalamic nuclei convey diverse contextual information to layer 1 of visual cortex
(a,b,c) Dots indicate cortical x-y positions of dLGN boutons (a), LP boutons (b) or V1 somata (c) within example imaged regions. In addition they are grayscale coded for receptive field position within the visual field with respect to relative elevation (top) or azimuth (bottom). Arrows indicate axes of cortical space that correlate best with changes in receptive field elevation (top) or azimuth (bottom). (d,e,f) Top, cortical axes for elevation (left) and azimuth (right) for all regions containing V1 cells, or dLGN or LP axons with at least 35 boutons. Bottom, distributions of separation angles between the two axes for elevation and azimuth for dLGN bouton populations (d), for LP bouton populations (e), and for V1 layer 2/3 neurons (f). For regions containing dLGN boutons, the directions of the axes were similar to those of V1 layer 2/3 neurons and the angles that separated the azimuth and elevation axes were close to 90° for all imaged sites (dLGN and V1: P-values < 0.05, Χ2-test for uniformity), showing a consistent fine-scale retinotopic organization. In contrast, the axes directions and separation angles varied considerably in regions containing LP boutons (P = 0.52, Χ2-test for uniformity), suggesting that LP input is not as closely aligned with the retinotopic map of V1 as dLGN input. M: medial, R: rostral, L: lateral, C: caudal. dLGN: n = 11 regions, 5 mice, LP: n = 13 regions, 7 mice, V1: n = 8 regions, 4 mice.
