Supplementary Figure 12: Spatial frequency tunings of LGN axons and V1 neurons.

(a-e) Distribution of preferred spatial frequency was shown as histograms for LGN axons in layer 1 (a, n = 3,817 boutons from 20 mice), 2/3 (b, n = 2,669 boutons from 18 mice) and 4 (c, n = 5,576 boutons from 20 mice) and V1 neurons in layer 2/3 (d, n = 7,635 neurons from 10 mice) and 4 (e, n = 6,210 neurons from 9 mice). Mean preferred frequency of layer 4 LGN axons (c) was statistically different from other layers’ axons (a, b) and V1 neurons (d, e) (p < 0.001 with layer 1 axons and V1 neurons, p < 0.05 with layer 2/3 axons, Mann-Whitney U-test with Bonferroni correction). (f) Proportion of SPF selective LGN axons and V1 neurons were not statistically different (p > 0.05, Mann-Whitney U-test with Bonferroni correction). Mean preferred SPF was significantly lower in layer 4 LGN axons than layer 4 V1 neurons, suggesting further computation in the cortical circuit.