Supplementary Figure 1: Morphology and firing pattern of DCX+ young and mature hippocampal GCs in slices from the adult mouse brain.
From: Bidirectional GABAergic control of action potential firing in newborn hippocampal granule cells
(a) Confocal z-projection of a biocytin-filled (green) newborn granule cell with an input resistance of 9 GΩ. Small images of immunohistochemical stainings show double-labeling with strong DCX expression (red). (b) Ca2+-spike and AP induced by somatic current injection in a 9 GΩ granule cell. Minimal current for AP induction is indicated. (c) Decay after a small hyperpolarization revealed slow membrane time constant (τm = 186 ms, monoexponential fit in red). (d) Same as a for a 3 GΩ granule cell showing slightly advanced dendritic maturation and weaker DCX expression. (e) Firing pattern of the cell in d. (f) Voltage decay shows faster membrane time constant (τm = 94 ms). (g) Morphology of a mature DsRed-DCX- granule cell. (h) Mature firing pattern for the cell in g requires high current injection. (i) Voltage decay after the current pulse revealed a fast τm of 23 ms.
