Supplementary Figure 4: β2*-nAChRs are not involved in motivation by certain rewards

(a) Learning of the task in the DS with increasing performance along learning sessions for both groups (session effect: F=12.16, p<0.001), which was not different between β2 KO and WT mice (genotype effect: F=0.04, p=0.84, genotype x session interaction: F=0.99, p=0.45, two-way ANOVA). (b) In the DS, the rate of ICSS behavior (number of ICSS per minute) scaled with the intensity of current pulses up to a plateau for both groups (intensity effect: p=<0.001, two-way ANOVA). When tested with different intensities of current pulses, β2KO mice performed the task with the same level of performance (genotype effect: F=1.22, p=0.27, genotype-intensity interaction: F=0.73, p=0.74, two-way ANOVA). (c) In the deterministic setting (DS), when the ICSS intensity increases (I(ICSS) = {40,80,120} µA), the speed profile of β2KO mice is affected, with higher maximal speed (F_(2,10)=36.35, p<0.001, one-way ANOVA) at higher intensities of ICSS. (d) Maximal speeds corresponding to the three ICSS intensities ({40,80,120} µA) for β2 KO and WT mice did not differ significantly (genotype effect: F=0,86, p=0,36; genotype x intensity interaction: F=0.16, p=0.86). Note that the values given in (d) do not correspond to the peaks in the speed profiles because the maximum of the average speed profile does not necessarily correspond to the average of the maximal speeds.