Supplementary Figure 5: PTEN recruitment to synaptic compartments
From: PTEN recruitment controls synaptic and cognitive function in Alzheimer's models
a. Confocal projection image of a primary hippocampal neuron (21 days in vitro) expressing EGFP-tagged WT (full-length) PTEN before (top) and 1 h after treatment with 4 μM synthetic Aβ42 assemblies (bottom). See Methods for preparation of primary neuronal cultures and imaging techniques. b. Quantification of time lapse imaging of the spine/dendrite ratio of WT EGFP-PTEN up-to 60 min after 1 μM Aβ42 application. N represents number of spines from 3 cells analyzed on 3 independent experiments. c. Top: Representative blot of PTEN amount on synaptosomal preparation prepared from hippocampal slices treated with Aβ for different periods of time. Uncropped versions of the western blots are shown in Supplementary Fig. 10. Bottom: Quantification of the blots represented at the top panel. N=5 rats.
