Supplementary Figure 6: Aβ overproduction does not globally alter the PIP₃ pathway

a. Representative blot of PTEN (left) and quantification of PTEN levels (right) from extracts of hippocampal slice cultures injected with App_swe/lnd–EGFP Sindbis virus and uninfected slices. b, c. Representative blot (left) and quantification (right) of phosphorylated and total AKT (b: pAKT(T308), tAKT), phosphorylated and total GSK3β (c: pGSK3β(S9), tGSK3β), and tubulin from extracts of hippocampal slice cultures injected with App_swe/lnd-EGFP Sindbis virus or control slices. d-f. Similar to a-c with extracts of hippocampi from App/Psen1 mice and their WT littermates. N represents the number of experiments. Statistical significance was calculated according to the Mann-Whitney test. g. Top: High magnification images of dendritic spines from neurons expressing WT EGFP-GSK3β. Bottom: Quantification of time lapse imaging of the spine/dendrite ratio of WT EGFP-GSK3β up-to 60 min after Aβ42 application. N represents number of spines from 3 cells analyzed on 3 independent experiments. h. Top: Representative blot of pAKT and tAKT amount on synaptosomal preparation prepared from hippocampal slices treated with Aβ for different periods of time. Bottom: Quantification of the blots represented at the top panel. N=6 rats. Uncropped versions of the western blots in panels a-f and h are shown in Supplementary Fig. 10.