Supplementary Figure 1: PCR-genotyping of the three mouse models used in this study and controls for behavioral experiments after semi-chronic Pten inhibition.
From: PTEN recruitment controls synaptic and cognitive function in Alzheimer's models
a-c. DNA from App/Psen1 (a), Ptentg (b) and PtenΔPDZ (c) was analyzed by PCR with the primers described in Supplemental Information. Representative PCR reactions from transgenic animals and wild-type littermates are shown for each case. d. Total time spent exploring in the novel object location task, by App/Psen1 animals and WT littermate controls, with or without VO-OHpic treatment. e. Percentage freezing displayed by App/Psen1 animals and WT littermate controls, with or without VO-OHpic treatment, before fear conditioning (white columns) or after contextual fear conditioning, before tone presentation in the memory test (see Methods). f. Representative western blot of hippocampal homogenates prepared from App/Psen1 mice and their WT littermates after semi-chronic (3-4 weeks) treatment with VO-OHpic. Uncropped version of the western blot is shown in Supplementary Fig. 10.
