Supplementary Figure 7: Newly introduced and specifically depleted CREs in humans.
From: Epigenomic annotation of gene regulatory alterations during evolution of the primate brain
(a) RPM normalized ChIP-seq read distribution (axis limit 5 RPM) for H3K27ac at a genomic region spanning ~173kb. Eight tracks represent the different anatomical brain regions that were analyzed in HS1, Ch1 and RM1 as indicated by the silhouettes. Colored boxes highlight CREs that are significantly higher in human (purple, 3 putative enhancers and 1 promoter), significantly lower in human (light brown, 1 enhancer) and gained in great apes (yellow, 1 enhancer in the subcortical structures). (b) Same analysis as (a) for a human-specific depletions. (c) Left graph shows distributions of human enhancers over the subdivisions of the brain. Fractions of shared enhancers between subdivisions are plotted in diagonal stripes that are colored according to the different subdivisions. This is shown for all predicted human enhancers and for newly introduced enhancers in comparison to rhesus macaque (random sampling, P < 0.001). Right graph shows fraction of new enhancers per (shared) anatomical subdivision of the original set of all enhancers. (d) Similar analysis as in (c) for human-specific depletions.
