Supplementary Figure 7: TAT-Panx308 is a specific inhibitor of Src-mediated activation of Panx1. | Nature Neuroscience

Supplementary Figure 7: TAT-Panx308 is a specific inhibitor of Src-mediated activation of Panx1.

From: Metabotropic NMDA receptor signaling couples Src family kinases to pannexin-1 during excitotoxicity

Supplementary Figure 7

Averaged NMDAR currents in response to 10µM NMDA puff. Recordings were in voltage clamp, holding at -60mV in 10µM glycine / 0mM Mg2+ aCSF. Bath applied TAT-Panx308 (1µM) treatments were compared against the Src inhibitor PP2 (10µM a known de-potentiator of NMDAR activity), and its inactive control PP3 (10µM). Data were quantified as peak amplitude elicited by NMDAR stimulation normalized to baseline. Bath application of 1 μM TAT-Panx308 did not reduce NMDAR currents, suggesting that TAT-Panx308 is not a general antagonist of Src.

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