Supplementary Figure 8: Panx1 opening in the plasma membrane induces mitochondrial dysfunction during ischemia.
From: Metabotropic NMDA receptor signaling couples Src family kinases to pannexin-1 during excitotoxicity
a) Images showing that calcein-green loading is colocalized with mitochondria specific stain, mitotracker red (upper panels). Co-loading of neurons with calcein green and calcein red/orange in the presence of CoCl2 allowed for simultaneous imaging of mPTP as green dye destaining and Panx1 opening as loss of the red dye from the cytosol. b) Families of raw data traces of calcein-green fluorescence showing mitochondrial destaining (decreased fluorescence) following the onset of OGD. c) Mean ± sem for calcein green fluorescence loss during OGD. d) Statistical comparison of mitochondrial destaining at 60 minutes following OGD application. e) Demonstration that the mPTP antagonist did not directly alter efflux of calcein red/orange from the cytosol through Panx1.
