Supplementary Figure 2: Chronic social defeat decreases c-fos expression in the LC of susceptible mice.
From: Resilience to chronic stress is mediated by noradrenergic regulation of dopamine neurons
(a) Illustration of c-fos DAB immunostained positive cells in the LC (left; scale bars: left 100 µm, right 50 µm). This staining has been reproduced on 12 mice. Quantification of c-fos positive cells in the LC (relative % to control) showed a significant decrease in susceptible mice (n=4) compared with control mice (n=3) (resilient mice n=4; F(2,7)=7.72, p=0.017; post-hoc **p=0.0057, right). (b) Illustration of retrograde tracer bilaterally injected into the VTA. (c) Representative photographs of the TH/c-fos/Beads triple labelling in the LC of a control mouse (left, scale bars: 50 µm) with high magnification (right, scale bars: 10 µm) of a c-fos/TH/Beads cell (upper, yellow arrow), a c-fos/Beads/NonTH cell (middle, red arrow) and a c-fos/TH/NonBeads cell (lower, green arrow). This staining has been reproduced on 24 mice. (d) Number of c-fos positive cells per µm2 in the LC, co-expressing or not TH and/or the beads retrograde markers injected into the VTA in control (n=9), susceptible (n=7) and resilient (n=8) mice to chronic social defeat (F(2,20)=5.8, p=0.015 ; post-hoc *p=0.005 in comparison with control and p=0.043 in comparison with resilient mice).
